| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042327 |
| Chromosome: | chromosome 5 |
| Location: | 3252250 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g240150 | UBC16 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACTAAAGCCCAGAGCTGCTCGCGGCCCCG |
| Internal bar code: | CTGGTGCGTGCTCGTAAGCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 99.0835 |
| LEAP-Seq n confirming: | 3027 |
| LEAP-Seq n nonconfirming: | 28 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCATTTTTGCTCCTGTT |
| Suggested primer 2: | GAAGCCCTCGCTTACAACTG |