Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | + |
Strain: | LMJ.RY0402.042328 |
Chromosome: | chromosome_4 |
Location: | 3044945 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g225200 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | ACAACAAATGGTGTGCGTGTGTGGGGGGGG |
Internal bar code: | GCACAGGTTCCCTCCGCTCAAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 589 |
LEAP-Seq percent confirming: | 99.6099 |
LEAP-Seq n confirming: | 17874 |
LEAP-Seq n nonconfirming: | 70 |
LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GGATCGCCGTACTACCGTAA |
Suggested primer 2: | ACACACACACACACACACGG |