| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042328 |
| Chromosome: | chromosome_4 |
| Location: | 3044962 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre04.g225200 | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TCAGCCCCGCCGCCCGCCCGCCCCTCACCC |
| Internal bar code: | TGGCCGTTGGGTGGATTCGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 370 |
| LEAP-Seq percent confirming: | 79.4503 |
| LEAP-Seq n confirming: | 607 |
| LEAP-Seq n nonconfirming: | 157 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAATGCCCCTTTTAACACGC |
| Suggested primer 2: | GGATCGCCGTACTACCGTAA |