| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042382 |
| Chromosome: | chromosome 2 |
| Location: | 7512589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144005 | (1 of 4) 2.3.1.43 - Phosphatidylcholine--sterol O-acyltransferase / Phospholipid--cholesterol acyltransferase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGGCCGCAAGCGCGCCAGGCAGCCAGCA |
| Internal bar code: | GCCGGCTGTTGTGGCCCCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 854 |
| LEAP-Seq percent confirming: | 89.7267 |
| LEAP-Seq n confirming: | 3808 |
| LEAP-Seq n nonconfirming: | 436 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCGGGTGTTGTTCAGGT |
| Suggested primer 2: | CAAATTGCGAAGCTTGAACA |