Insertion junction: LMJ.RY0402.042385_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.042385
Chromosome:chromosome 10
Location:1817286
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g431050 SRR13 (1 of 1) PTHR19331//PTHR19331:SF253 - LYSYL OXIDASE-RELATED // SUBFAMILY NOT NAMED; Speract/scavenger receptor, transmembrane glycoprotein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTGCGTGGCGGGGTACGACGTCAACGGCC
Internal bar code:TTGCATATAGGATTCGTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:512
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AATATCCCGCTGAACAGCAC
Suggested primer 2:GTTTGCCCTGACTGAAGCTC