| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042397 |
| Chromosome: | chromosome 16 |
| Location: | 627560 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g692000 | (1 of 9) 3.1.3.2 - Acid phosphatase / Phosphomonoesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGAGGAGTATCTGTCGCGTGTGGTCGGCGG |
| Internal bar code: | AGGATTACTAAGTTATCAGACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 645 |
| LEAP-Seq percent confirming: | 78.9727 |
| LEAP-Seq n confirming: | 492 |
| LEAP-Seq n nonconfirming: | 131 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCAATGCCCGAGTAAGG |
| Suggested primer 2: | AGTCCACCACCGAGTACAGG |