| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042412 |
| Chromosome: | chromosome 12 |
| Location: | 5740441 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533000 | PHR2,PHR1 | (1 of 3) K01669 - deoxyribodipyrimidine photo-lyase [EC:4.1.99.3] (phrB); CPD photolyase, class II | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCCCACCCCCGCCGCTCCGAGCGACGCAG |
| Internal bar code: | TTTTCCTTTGGATACGCTACAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 342 |
| LEAP-Seq percent confirming: | 99.5233 |
| LEAP-Seq n confirming: | 5011 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGAACTACAACGGCTGCAAG |
| Suggested primer 2: | GCATCCTGACTCGGAAAGAG |