| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042416 |
| Chromosome: | chromosome 9 |
| Location: | 942103 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401000 | SNE3 | UDP-N-acetylglucosamine/UDP-glucose 4-epimerase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACGCTCGCTCGCTCGCTCGCTCGCTCGC |
| Internal bar code: | TAGGCCCCTGCTCGATGCGCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 62 |
| LEAP-Seq percent confirming: | 97.1264 |
| LEAP-Seq n confirming: | 169 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAAGGAGGTGGGAAAGGAG |
| Suggested primer 2: | GTATGGGTGCAGGTGAAGGT |