| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042433 |
| Chromosome: | chromosome 16 |
| Location: | 1235954 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651000 | RFA3,RPA70B | (1 of 1) PTHR23273:SF4 - REPLICATION PROTEIN A 70 KDA DNA-BINDING SUBUNIT; Replication protein A, 70 kDa DNA-binding subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTCCCTTTGCCCCACCTTGAACTTGTGGA |
| Internal bar code: | CTTTGCCACTCTGCGGCTGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 478 |
| LEAP-Seq percent confirming: | 99.8596 |
| LEAP-Seq n confirming: | 1423 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATAATGCACATGGCACG |
| Suggested primer 2: | CGTTTGGAAGATGGACAGGT |