| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042476 |
| Chromosome: | chromosome 9 |
| Location: | 691589 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g402900 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGCAGCCCACGCGCAAGCTCCGGTACGC |
| Internal bar code: | CCTTGCGGCCATGCAATAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 98.8648 |
| LEAP-Seq n confirming: | 4964 |
| LEAP-Seq n nonconfirming: | 57 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAAGAGCCACGTCTCCAGTC |
| Suggested primer 2: | GGCCAAAGCTATGCTACGAG |