| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042478 |
| Chromosome: | chromosome_3 |
| Location: | 4766007 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g178150 | RSP20,CAM1 | Calmodulin, radial spoke protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCCCGGGTTACACATAGGGGCGTGCTGTC |
| Internal bar code: | CTTTCTGGCTAAAGTGTGATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 98.9435 |
| LEAP-Seq n confirming: | 20790 |
| LEAP-Seq n nonconfirming: | 222 |
| LEAP-Seq n unique pos: | 140 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGAAGGCTGGAGTTCAC |
| Suggested primer 2: | ATTGACCGTGTCCTACCTGC |