| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042478 |
| Chromosome: | chromosome 3 |
| Location: | 4766007 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g178150 | RSP20,CAM1 | Calmodulin, radial spoke protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGTTCCTGCTACGTGTTTCAATCCCTG |
| Internal bar code: | AGCTCGTACCGTGCGTGGCTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1555 |
| LEAP-Seq percent confirming: | 99.4904 |
| LEAP-Seq n confirming: | 33583 |
| LEAP-Seq n nonconfirming: | 172 |
| LEAP-Seq n unique pos: | 38 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTGAAGGCTGGAGTTCAC |
| Suggested primer 2: | ATTGACCGTGTCCTACCTGC |