| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042488 |
| Chromosome: | chromosome 10 |
| Location: | 1586709 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429350 | (1 of 6) 3.4.22.69 - SARS coronavirus main proteinase / Severe acute respiratory syndrome coronavirus main protease | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTCGTGGCGGCGTCGCGCGAGCTGGTGGA |
| Internal bar code: | CCGTGTTAGGCGAATCTCGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 170 |
| LEAP-Seq percent confirming: | 49.7994 |
| LEAP-Seq n confirming: | 2606 |
| LEAP-Seq n nonconfirming: | 2627 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGGCCTTCTCCTTCTTCT |
| Suggested primer 2: | ATCTTTGAGCTGTGGGCACT |