| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042496 |
| Chromosome: | chromosome 7 |
| Location: | 1432894 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g323050 | PEN2 | Presenilin enhancer | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTGATCATTACCAACCGCCAATCACTG |
| Internal bar code: | TCTGCCATGGGGCAAGGCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 523 |
| LEAP-Seq percent confirming: | 99.5261 |
| LEAP-Seq n confirming: | 1890 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCTTACAGGGGTAACAGG |
| Suggested primer 2: | TGTAGCTGTAGGTGCGTTGC |