| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042500 |
| Chromosome: | chromosome 12 |
| Location: | 7979820 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552100 | (1 of 15) 3.6.5.5 - Dynamin GTPase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCATAACCCAGTCATGCATGCGGGCAGCTG |
| Internal bar code: | CCAGATTAGGGCGCATCCGACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 806 |
| LEAP-Seq percent confirming: | 99.1071 |
| LEAP-Seq n confirming: | 888 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTCGGCTGTGGTAAGTAT |
| Suggested primer 2: | CAAGGAGGAGGAGCAAACTG |