| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042509 |
| Chromosome: | chromosome 7 |
| Location: | 5158985 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348250 | (1 of 1) K14840 - nucleolar protein 53 (NOP53, GLTSCR2) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGAGGTCAGCTAGGTGTGGTACTGCTGATG |
| Internal bar code: | CGCCCTGGATGTGGCTTGGCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 890 |
| LEAP-Seq percent confirming: | 98.2394 |
| LEAP-Seq n confirming: | 3627 |
| LEAP-Seq n nonconfirming: | 65 |
| LEAP-Seq n unique pos: | 53 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTGGTTTAGTTTGGGCTGG |
| Suggested primer 2: | GGCCGACTGAATGTACCAGT |