| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042528 |
| Chromosome: | chromosome 6 |
| Location: | 2547097 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g269250 | FKM5 | DNA methylase in the FkbM family; (1 of 13) PF05050 - Methyltransferase FkbM domain (Methyltransf_21) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACACAGCCTCACGGACACAGCCTCACAG |
| Internal bar code: | CATACCTCCGAACTCGGAGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 231 |
| LEAP-Seq percent confirming: | 96.4047 |
| LEAP-Seq n confirming: | 1877 |
| LEAP-Seq n nonconfirming: | 70 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGGGTAATGTCTTGTTGCT |
| Suggested primer 2: | TCACTGGGTTTCCTGGATTC |