| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042531 |
| Chromosome: | chromosome_6 |
| Location: | 2388383 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g267850 | PLS1 | Phospholipid scramblase | antisense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TGTTCCAGCGCTGGTGGATCTCACCCAGCT |
| Internal bar code: | GGTCGGGACCTGGTTCGCGGCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 848 |
| LEAP-Seq percent confirming: | 97.7117 |
| LEAP-Seq n confirming: | 4996 |
| LEAP-Seq n nonconfirming: | 117 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAGTGTGGATACCATGCAG |
| Suggested primer 2: | TGAGCGTGTGTGTTAGGGAG |