| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042549 |
| Chromosome: | chromosome 2 |
| Location: | 2480653 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g092000 | DEG2 | Deg protease; (1 of 5) 3.4.21.108 - HtrA2 peptidase / Serine proteinase OMI | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCCTGCTATTTATCTGCCCTCGGCGCCGG |
| Internal bar code: | TTCTTTATTGTAAGTCGCTCTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 263 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 210 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGAATTGGTGGGTAAGA |
| Suggested primer 2: | TAATCCGGTGATGCTGATGA |