Insertion junction: LMJ.RY0402.042578_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.042578
Chromosome:chromosome 3
Location:7809006
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre03.g203000 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGTCACGTGTTGCTCGTTTCTTAGCAGT
Internal bar code:AACCGAAAACACGCCACGCTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:283
LEAP-Seq percent confirming:99.3321
LEAP-Seq n confirming:4164
LEAP-Seq n nonconfirming:28
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCAAACGAAGACACCTAA
Suggested primer 2:CGGGGTAGAGCTTGTGGTAA