| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042582 |
| Chromosome: | chromosome 12 |
| Location: | 8324565 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g549650 | (1 of 89) 3.6.4.13 - RNA helicase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTCGTGCTGGGCTGGGCGTGTTGGCTGG |
| Internal bar code: | GTGGGAGAGTGTATACGAAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 836 |
| LEAP-Seq percent confirming: | 88.9463 |
| LEAP-Seq n confirming: | 3098 |
| LEAP-Seq n nonconfirming: | 385 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTCGTCGAAAACCTGC |
| Suggested primer 2: | TGATGATGTGGGAGTCAGGA |