| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042594 |
| Chromosome: | chromosome 9 |
| Location: | 4228717 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g394399 | (1 of 1) K14767 - U3 small nucleolar RNA-associated protein 3 (UTP3, SAS10) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGAATAAGATTGGCCCCTCCTACAAGACC |
| Internal bar code: | CTACCTTCTTGGGCCCCTTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 534 |
| LEAP-Seq percent confirming: | 89.0315 |
| LEAP-Seq n confirming: | 3815 |
| LEAP-Seq n nonconfirming: | 470 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCGCATACCACCCCATATC |
| Suggested primer 2: | GAGGAGTATGACCTCAGCGG |