| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042595 |
| Chromosome: | chromosome 2 |
| Location: | 5113525 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105000 | (1 of 3) PTHR11207//PTHR11207:SF7 - RIBONUCLEASE III // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTGTCGCCTCCTGTCTCCCCCTGACTCA |
| Internal bar code: | TCTACCAAGCGTCCTGGGGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 568 |
| LEAP-Seq percent confirming: | 99.2997 |
| LEAP-Seq n confirming: | 709 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGGTATCGAAACTTCGTC |
| Suggested primer 2: | GTTGAGGGTGTGTAGGCGTT |