| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042599 |
| Chromosome: | chromosome 17 |
| Location: | 2080210 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g712100 | MDAR1,PNO2 | Monodehydroascorbate reductase 1; (1 of 1) K08232 - monodehydroascorbate reductase (NADH) (E1.6.5.4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTGGGGTCCACGGCGTGCACTTTAGGGG |
| Internal bar code: | GAATCAATGTCCTAGATCCGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 833 |
| LEAP-Seq percent confirming: | 98.9588 |
| LEAP-Seq n confirming: | 10360 |
| LEAP-Seq n nonconfirming: | 109 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTCTGGTTAGCGACCTGTG |
| Suggested primer 2: | GGGGTAGGAGGTTTAGCGTC |