| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042615 |
| Chromosome: | chromosome 3 |
| Location: | 5220692 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182750 | (1 of 10) 3.1.1.5 - Lysophospholipase / Phospholipase B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGGACGGCAAGATGTTGCCGGCGCGG |
| Internal bar code: | AGTTATCAAGGCGACAATATAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 985 |
| LEAP-Seq percent confirming: | 99.3978 |
| LEAP-Seq n confirming: | 2476 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCATAGGTTCTGCCACT |
| Suggested primer 2: | TGACCTTGGAGTCTGCAGTG |