| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042628 |
| Chromosome: | chromosome 17 |
| Location: | 2286076 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g713850 | SAT2,DIV184 | (1 of 1) PTHR23416:SF63 - SERINE ACETYLTRANSFERASE 2-RELATED; Serine O-acetyl transferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTAGCGCAGCCAACACACGCTCTAACCCTT |
| Internal bar code: | TGTCAATTTTTCAATTATATCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 102 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGCACGGAGTGAGGTTAT |
| Suggested primer 2: | CGTTTGTTTTCTGTTCGGGT |