Insertion junction: LMJ.RY0402.042638_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.042638
Chromosome:chromosome 12
Location:725062
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre12.g495100 PSR1 (1 of 1) PF00249//PF14379 - Myb-like DNA-binding domain (Myb_DNA-binding) // MYB-CC type transfactor, LHEQLE motif (Myb_CC_LHEQLE); Phosphorus starvation response 1 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGCAGTTAGGACGCCGTACGCCATAGAT
Internal bar code:AGGTCGAATCTCATTTTTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:305
LEAP-Seq percent confirming:99.5406
LEAP-Seq n confirming:3467
LEAP-Seq n nonconfirming:16
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ATGCCCGTTTGCATAAAGTC
Suggested primer 2:CCTGTCCCATCACACACTTG