| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042654 |
| Chromosome: | chromosome 1 |
| Location: | 5260354 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036700 | (1 of 4) PTHR24347:SF138 - PROTEIN F32D8.1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGAACCCAATAGCACGCACCATACCCGCG |
| Internal bar code: | CGACACAGCACTCTTAACGCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1071 |
| LEAP-Seq percent confirming: | 95.1358 |
| LEAP-Seq n confirming: | 9916 |
| LEAP-Seq n nonconfirming: | 507 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTCTAGCTAACGACCCGCTG |
| Suggested primer 2: | CTAAGAGCCCTGCATTCTGC |