Insertion cassette: | CIB1 |
Side of cassette: | 5' |
Strand: | - |
Strain: | LMJ.RY0402.042655 |
Chromosome: | chromosome_8 |
Location: | 3499759 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre08.g375050 | FAP86 | Flagellar Associated Protein | sense | CDS |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AATGAGCTTGGCGGCGGGGCCCGCTGGCAC |
Internal bar code: | GTTTACTCATGTTCGTACGAGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 521 |
LEAP-Seq percent confirming: | 99.9195 |
LEAP-Seq n confirming: | 1242 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TTCGAGTTGTCATTTGAGCG |
Suggested primer 2: | TACTTGGAGCCGATGAGCTT |