| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042659 |
| Chromosome: | chromosome 12 |
| Location: | 2979033 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g501200 | SKP1,ROC80 | E3 ubiquitin ligase; (1 of 1) K03094 - S-phase kinase-associated protein 1 (SKP1, CBF3D) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTGCAAGCAGCATGCCCACCGTTGGCAC |
| Internal bar code: | ATAGAGGGCAGAGGGGGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 839 |
| LEAP-Seq percent confirming: | 99.2248 |
| LEAP-Seq n confirming: | 896 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGGAACCTGTCACGAGT |
| Suggested primer 2: | TGGCTCAGATGATTAAGGGG |