| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042697 |
| Chromosome: | chromosome 4 |
| Location: | 1213096 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g215400 | AKC3 | conserved expressed protein related to ABC1/COQ8 mitochondrial putative ser/thr kinase; (1 of 2) PTHR10566//PTHR10566:SF86 - CHAPERONE-ACTIVITY OF BC1 COMPLEX CABC1 -RELATED // ABC1 PROTEIN KINASE 6 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATCCGTGGTCAGCTGTGGTGAGGTGGGAG |
| Internal bar code: | GGTGGCCTACTCAGGGGTCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 181 |
| LEAP-Seq percent confirming: | 98.1818 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACCACCTACACGTGAC |
| Suggested primer 2: | CATCCGACGACTGGGTATCT |