| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042706 |
| Chromosome: | chromosome 1 |
| Location: | 2014427 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010848 | (1 of 1) K06997 - uncharacterized protein (K06997) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCAGGCGGTGAGGAGCGAGGAGAGGGAGG |
| Internal bar code: | GTTAGAGGTTACTGGAAGTTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 675 |
| LEAP-Seq percent confirming: | 99.6235 |
| LEAP-Seq n confirming: | 1323 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCGCCCACTTCTCTACCTG |
| Suggested primer 2: | GTCCCAACCCTTCTCTCTCC |