Insertion junction: LMJ.RY0402.042709_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.042709
Chromosome:chromosome 3
Location:2286413
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre03.g158100 SMM9 (1 of 1) IPR026170//IPR029063 - FAM173 family // S-adenosyl-L-methionine-dependent methyltransferase; S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTCATCGCACTCGAACGCACAGGTCATCA
Internal bar code:GTCGCACCCATCGTCAATGTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:87
LEAP-Seq percent confirming:5.88235
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TGGCAGCACATATACGGTGT
Suggested primer 2:AATGGGGGCATCAAACAATA