| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042715 |
| Chromosome: | chromosome 5 |
| Location: | 326548 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g233300 | (1 of 1) 2.1.1.98 - Diphthine synthase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAGATTCGGGGAGGAGAGATGTGTGTGT |
| Internal bar code: | TGCCCCGTGGCAGGTTTCAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 285 |
| LEAP-Seq percent confirming: | 98.3747 |
| LEAP-Seq n confirming: | 2300 |
| LEAP-Seq n nonconfirming: | 38 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCCAGTCACCCTCACTTG |
| Suggested primer 2: | CCCTCAGGAGAAGCTGTACG |