| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042719 |
| Chromosome: | chromosome_7 |
| Location: | 940466 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g319150 | PTP4 | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | TTGGGAAGCCGGAGGTGGCGGCGGTGGTGG |
| Internal bar code: | TGCGTGTTCCATCAACTTGCAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 646 |
| LEAP-Seq percent confirming: | 92.3077 |
| LEAP-Seq n confirming: | 12 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAAGTTGCTCTGTGCTTC |
| Suggested primer 2: | TAACCCCACACACACACACC |