| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.042730 |
| Chromosome: | chromosome 10 |
| Location: | 2726616 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438300 | LTH4 | (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease; LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGTTTGACTTGGCTGAAAGCATGCGAAT |
| Internal bar code: | GCGTGGCTCGCTCAATGGACGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1058 |
| LEAP-Seq percent confirming: | 99.8327 |
| LEAP-Seq n confirming: | 2387 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 8 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTACGGTGCCACAGGTCTT |
| Suggested primer 2: | TGATCTAGTGCTGCTGGTCG |