Insertion junction: LMJ.RY0402.042733_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.042733
Chromosome:chromosome 7
Location:3798549
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g338451 FBP1,GLPX1 Fructose-1%252C6-bisphosphatase Type II; (1 of 1) PTHR30447 - FRUCTOSE-1,6-BISPHOSPHATASE CLASS 2 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTGTTCGTGCCTTTCGGAAGGCCCGTCAAA
Internal bar code:TCTCGTCGCTACTCCGCGGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:1010
LEAP-Seq percent confirming:89.7732
LEAP-Seq n confirming:10332
LEAP-Seq n nonconfirming:1177
LEAP-Seq n unique pos:51

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAGGTCCACCACTACACC
Suggested primer 2:AATCCACACGAACACACGAA