| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.042762 |
| Chromosome: | chromosome 2 |
| Location: | 2253595 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g090100 | (1 of 1) PTHR22883//PTHR22883:SF23 - ZINC FINGER DHHC DOMAIN CONTAINING PROTEIN // PALMITOYLTRANSFERASE ZDHHC4-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTGTATGGCTATCCGTGTCTTGTGTGCGC |
| Internal bar code: | TAGATGGGCAGGTCGGAGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 641 |
| LEAP-Seq percent confirming: | 55.0191 |
| LEAP-Seq n confirming: | 7942 |
| LEAP-Seq n nonconfirming: | 6493 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTGGTGAAACGTCTGGGAG |
| Suggested primer 2: | GCTGGTGTGCGTGATAGGTA |