| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042815 |
| Chromosome: | chromosome 7 |
| Location: | 4149652 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g342550 | SMM28 | S-adenosyl-L-methionine-dependent methyltransferase; (1 of 1) PTHR10108:SF827 - METHYLTRANSFERASE-LIKE PROTEIN-RELATED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGATTCAGTGCAGGCTCGCAGATGGCAAAA |
| Internal bar code: | GCCGCCGATGAATTTTCACCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 602 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 20 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGATCGGATTGCAGGAGT |
| Suggested primer 2: | ACACACACACACACACACCG |