Insertion junction: LMJ.RY0402.042835_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.042835
Chromosome:chromosome 2
Location:401041
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g076000 LAT3,STK1,STPK1,SNRK2K (1 of 587) 2.7.11.1 - Non-specific serine/threonine protein kinase / Threonine-specific protein kinase; snRK1 family in Chlamydomonas, subgroup 2; Latrunculin-B sensitive 3 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAACCCGCCTCCGGCTAGTCACTGCTGC
Internal bar code:GCGCTTTCTGTGAACGACTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:780
LEAP-Seq percent confirming:6.99405
LEAP-Seq n confirming:94
LEAP-Seq n nonconfirming:1250
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CACCTCTAAAGGCTTCACCG
Suggested primer 2:CACGACGCATTTTCAATGTC