| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042847 |
| Chromosome: | chromosome_7 |
| Location: | 3786411 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g338350 | CPLD50 | Predicted protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGCTCGGGCACATACTCCTAGGCTACAACT |
| Internal bar code: | CTGGTGACCCGGAAAAATCGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 223 |
| LEAP-Seq percent confirming: | 99.3311 |
| LEAP-Seq n confirming: | 1188 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAGGACTGGGTACTGGGTA |
| Suggested primer 2: | CGTGGTTTTATTGCGTGTTG |