| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.042856 |
| Chromosome: | chromosome 11 |
| Location: | 470532 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467592 | Probable amino acid/metabolite permease; (1 of 1) PTHR11785//PTHR11785:SF228 - AMINO ACID TRANSPORTER // GABA-SPECIFIC PERMEASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCATGCACATCTAGCACCCAACACAGAAAG |
| Internal bar code: | ATCGGCGGAGTTTCCGCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 159 |
| LEAP-Seq percent confirming: | 99.6068 |
| LEAP-Seq n confirming: | 1520 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTTGGACCCTTGACGAGT |
| Suggested primer 2: | TGACACGGTTTCCGTACAAA |