Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.042907 |
Chromosome: | chromosome_12 |
Location: | 4125978 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g518050 | FAP261 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CAAGTATGGAGTGCCCACGTCCAGTAGCTA |
Internal bar code: | GCACAAGTGGCGATGGTGCTAG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 462 |
LEAP-Seq percent confirming: | 99.3438 |
LEAP-Seq n confirming: | 1968 |
LEAP-Seq n nonconfirming: | 13 |
LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | AACCTGATCTACACGCTGGG |
Suggested primer 2: | AACATTTTCGGAGTGATGGC |