Insertion junction: LMJ.RY0402.042917_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.042917
Chromosome:chromosome 1
Location:5420268
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre01.g038050 COP7,HKR3,COP Histidine kinase rhodopsin 3; (1 of 1) PF00072//PF00512//PF01036 - Response regulator receiver domain (Response_reg) // His Kinase A (phospho-acceptor) domain (HisKA) // Bacteriorhodopsin-like protein (Bac_rhodopsin) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATTTTACTAGTCGGGGCAGTTGTTATACA
Internal bar code:AGATTCATTGATGTAAACCACC

Confirmation - LEAP-Seq

LEAP-Seq distance:333
LEAP-Seq percent confirming:27.2684
LEAP-Seq n confirming:1136
LEAP-Seq n nonconfirming:3030
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTCCGACCTGTTTTACACCC
Suggested primer 2:AGCACACTGGTGAGCATGAG