| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.042945 |
| Chromosome: | chromosome 1 |
| Location: | 7393729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g053150 | GPDH3,GPD3 | (1 of 3) K00006 - glycerol-3-phosphate dehydrogenase (NAD+) (GPD1); Glycerol-3-phosphate dehydrogenase/dihydroxyacetone-3-phosphate reductase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATAGATGACATCCCCAAAGCAGGGCACTT |
| Internal bar code: | TTTCACGTATCCTTTCTTCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 505 |
| LEAP-Seq percent confirming: | 99.4243 |
| LEAP-Seq n confirming: | 2418 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCAACTCATGTGACGGG |
| Suggested primer 2: | ACACACACACACACACACCG |