Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.043008 |
Chromosome: | chromosome_16 |
Location: | 6895993 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre16.g676533 | sense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CTAGGGTCTGTGCTTGCGGGTGGGTACAAG |
Internal bar code: | GGACACAAGGGTGGGGGAGGGA |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 872 |
LEAP-Seq percent confirming: | 99.8058 |
LEAP-Seq n confirming: | 514 |
LEAP-Seq n nonconfirming: | 1 |
LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | ATGGTGAAGTAAAGTGGCGG |
Suggested primer 2: | CTCTGGACTGCTCCTTCCAC |