| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.043021 |
| Chromosome: | chromosome 13 |
| Location: | 4464035 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603450 | (1 of 4) PF14159 - CAAD domains of cyanobacterial aminoacyl-tRNA synthetase (CAAD) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGGAGGAGGCGGAGGGGTCGGGAATGC |
| Internal bar code: | ACCGCTGGAGCATTTGCAGCCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 331 |
| LEAP-Seq percent confirming: | 57.9832 |
| LEAP-Seq n confirming: | 207 |
| LEAP-Seq n nonconfirming: | 150 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGGCGGGGTTTCTCATT |
| Suggested primer 2: | GGTGGGTGTGGATGTAAAGG |