| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.043028 |
| Chromosome: | chromosome 6 |
| Location: | 5317218 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g283100 | FAP85 | Flagellar Associated Protein 85; (1 of 140) IPR002048 - EF-hand domain | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACATAAGCTGCGCAAGCGGGTGTACAT |
| Internal bar code: | CCGGTACGGAGGTACTGCGAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 619 |
| LEAP-Seq percent confirming: | 91.1401 |
| LEAP-Seq n confirming: | 5308 |
| LEAP-Seq n nonconfirming: | 516 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGTCAACTGGACTGGGAG |
| Suggested primer 2: | TCGTGCTAGATGCAGAGGTG |