| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043029 |
| Chromosome: | chromosome 17 |
| Location: | 3255715 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g722800 | MPPA1,MAS2 | Mitochondrial processing peptidase alpha subunit; (1 of 1) PTHR11851//PTHR11851:SF132 - METALLOPROTEASE // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTGATGTGAGCCGTGTGCACAGCTCGCAC |
| Internal bar code: | CTGTTGTGGGCCGCCTTAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 99.6606 |
| LEAP-Seq n confirming: | 5286 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACGTGGAGATGCTGGAGG |
| Suggested primer 2: | TGACACCTTCTGTAGCACCG |