| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.043031 |
| Chromosome: | chromosome 5 |
| Location: | 3414294 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241202 | (1 of 3) 3.4.22.53 - Calpain-2 / Milli-calpain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCGGCCGGTACTCGGAGCCCGACAGGAAC |
| Internal bar code: | GGGCGGTGTCACGAGCGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 458 |
| LEAP-Seq percent confirming: | 94.3836 |
| LEAP-Seq n confirming: | 4571 |
| LEAP-Seq n nonconfirming: | 272 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAATTCTGAAGGGCAGGGT |
| Suggested primer 2: | CTTCTCCAGCAGTAGCACCC |